CTrapPy configuration

Type: object

Configuration file for CTrapPy scan processing.

root initial_bead_fit
Type: array

Array of 3 numbers for automatic bead fitting; expected amplitude (ADU), left edge of left bead (px), right edge of left bead (px), left edge of right bead (px), right edge of right bead (px), avg background signal (ADU).

root colocalization_distance
Type: number

Colocalization distance in microns.

root diff_estimate
Type: number

Diffusion coefficient estimate in micron^2/s; needed for diffusion calculation.

root t_exposure
Type: number

Estimate of exposure time for a spot in seconds; needed for diffusion calculation.

root sigma_psf
Type: number

Estimate of PSF size in microns; needed for diffusion calculation.

root step_counting_divide
Type: boolean

Do we divide detected bleaching steps by the average expected step size?

root confocal_pixel_size
Type: number

Confocal pixel size in microns.

root min_step_sizes_r
Type: array

Array of 3 numbers; minimum step counts for R, G, B if R laser is on.

root min_step_sizes_g
Type: array

Array of 3 numbers; minimum step counts for R, G, B if G laser is on.

root min_step_sizes_b
Type: array

Array of 3 numbers; minimum step counts for R, G, B if B laser is on.

root min_step_sizes_rg
Type: array

Array of 3 numbers; minimum step counts for R, G, B if R, G lasers are on.

root min_step_sizes_rb
Type: array

Array of 3 numbers; minimum step counts for R, G, B if R, B lasers are on.

root min_step_sizes_gb
Type: array

Array of 3 numbers; minimum step counts for R, G, B if G, B lasers are on.

root min_step_sizes_rgb
Type: array

Array of 3 numbers; minimum step counts for R, G, B if R, G, B lasers are on.

root min_final_step_size
Type: array

Array of 3 numbers; minimum step counts for R, G, B if R, G, B lasers are on.

root avg_step_sizes_r
Type: array

Array of 3 numbers; average step counts for R, G, B if R laser is on.

root avg_step_sizes_g
Type: array

Array of 3 numbers; average step counts for R, G, B if G laser is on.

root avg_step_sizes_b
Type: array

Array of 3 numbers; average step counts for R, G, B if B laser is on.

root avg_step_sizes_rg
Type: array

Array of 3 numbers; average step counts for R, G, B if R, G lasers are on.

root avg_step_sizes_rb
Type: array

Array of 3 numbers; average step counts for R, G, B if R, B lasers are on.

root avg_step_sizes_gb
Type: array

Array of 3 numbers; average step counts for R, G, B if G, B lasers are on.

root avg_step_sizes_rgb
Type: array

Array of 3 numbers; average step counts for R, G, B if R, G, B lasers are on.

root bleed_through
Type: array

3x3x3 array; crosstalk matrices.